Aromatic composition of the, Rosilllo, L.; Salinas, M.R. Significant amounts of information on apple fruit volatile production during fruit ripening has been reported (Echeverría et al., 2004, Mattheis, 1991, Song and Bangerth, 1996). (2010). doi: 10.1021/jf010924l, Luciardi, M. C., Blázquez, M. A., Cartagena, E., Bardón, A., and Arena, M. E. (2016). J. Essent. Comprehensive Natural Products Chemistry: Isoprenoids Including Carotenoids and Steroids, Help us to further improve by taking part in this short 5 minute survey, The Application of Transient-State Kinetic Isotope Effects to the Resolution of Mechanisms of Enzyme-Catalyzed Reactions, Volatile Aroma Compounds in Various Brewed Green Teas, Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins, Supercritical Fluid Extraction of Plant Flavors and Fragrances, https://doi.org/10.3390/molecules18078200, Ogundiwin, E.A. J. Agric. doi: 10.1016/S0308-8146(03)00013-X, Alonzo, G., Fatta del Bosco, S., Palazzolo, E., Saiano, F., and Tusa, N. (2000). Fancello, F., Petretto, G. L., Zara, S., Sanna, M. L., Addis, R., Maldini, M., et al. Soc. Biochem. Vous reprenez donc la main, et vous ôtez 1 à ce nombre, il restera ainsi 68 qui est le double du nombre pensé ; soit 68 : 2 = 34. Applying this criterion, we observed that slightly more than half of these 763 reliably identified compounds are terpenoids, whilst the other half corresponds to a wide range of non-terpenoid compounds. Flavour Frag. There is also a group of non-terpenoid aliphatic and olefinic compounds frequently reported in several Citrus flowers, but not in rinds or leaves, including acetone (Jabalpurwala et al., 2009; Cheong et al., 2011b), isopropanol (Jabalpurwala et al., 2009), (Z)-jasmone (Jabalpurwala et al., 2009; Flamini and Cioni, 2010; Dugo et al., 2011; Družić, 2016), or pentadecane (Jabalpurwala et al., 2009; Flamini and Cioni, 2010; Zakaria et al., 2010). ; Mesa, J. Volatile fraction composition and biological activity of lemon oil (Citrus limon L. The non-volatile residue is mainly composed of flavonoids, coumarins, diterpenoids, sterols, and fatty acids. Forty-nine volatile organic compounds have been reported in all 10 species, most of them terpenoid (90%), although about half of the volatile compounds identified in Citrus peel are non-terpenoid. Most of the papers use shorter non-systematic names, but this results in many synonyms for the same compound. Food Chem. Hemiterpenes (C, In plastids, DMAPP generated from the MEP pathway is used by isoprene synthases for isoprene formation [, Many of the terpene volatiles are direct products of terpene synthases, while others are formed through alterations of the primary terpene skeletons made by TPSs by hydroxylation, dehydrogenation, acylation, and other reaction types [, Carotenoid and their apocarotenoid derivatives are isoprenoid molecules important for the primary and secondary metabolisms of plants and other living organisms [. ; Olivas, G.I. The bottles should be kept out of direct sunlight. It is well known that even though food products usually contain several hundreds of volatiles, most of them do not possess aroma activity in the existing concentration in the product. Inafuku-Teramoto, S., Suwa, R., Fukuzawa, Y., and Kawamitsu, Y. J. Sci. Kumar R, Sharma MK, Kapoor S, Tyagi AK, Sharma AK. “Minor” tropical fruit mango, papaya, passion fruit, and guava. ; Wang, Y.; Lin, J.M. RIPENING INHIBITOR (RIN)-deficient fruits generated by CRISPR/Cas9 initiated partial ripening at a similar time to wild-type (WT) fruits but only 10% WT concentrations of carotenoids and ethylene (ET) were synthesized. Thus, the EOs obtained from Citrus rinds always show limonene, a hydrocarbon monoterpene, as the most abundant compound, its concentration generally representing about 60–95% of the oil (Jing et al., 2014). doi: 10.1080/10412905.2011.9700445. Wyllie, S.G.; Fellman, J.K. Biochem. 28, 265–271. ex Tanaka). Volatile composition and odour-activity value of thornless “Black Diamond” and “Marion” blackberries. 4ème et dernière énigme de l'Elfe Chouqui Et voilà, Noël est à nos portes et la dernière énigme ainsi que son prix de l'Elfe Chouqui arrivent 2 Bons-Cadeaux pour le Brunch du Boulanger à Crissier d'une valeur de Fr. Trouvé à l'intérieur – Page 12759 . et redoublez chaque syllabe , vous aurez les noms de deux oiseaux ( 49 ) ; Énigme sur le mot Batiku ( melon ) : Trouvez un mot qui est le nom d'un fruit charmant ; sa première moitié est le nom d'un volatile , et le reste ... It also provides the possibility of developing species biomarkers based on this type of compounds, and points toward those which could be useful for the industry to monitor quality and consumer safety (Salgueiro et al., 2010). Constituents of the distilled essential oils of Citrus reticulata and C. paradisi from Nigeria. Thus, the employment of GC hyphenated to carbon isotope ratio mass spectrometry (GC-C-IRMS) can detect compounds of different botanical origin such as, for example, the presence of citral extracted from lemon grass (Cymbopogon citratus) (Schipilliti et al., 2018) in lemon EO. (2015), several compounds have been exclusively isolated in C. limon and C. grandis peels, specially some non-terpenoid aliphatic ester compounds such as (Z)-3-hexenyl hexanoate (Sun et al., 2014a; Cannon et al., 2015) and methyl octanoate, methyl nonanoate and methyl decanoate (Cheong et al., 2011b; Cannon et al., 2015). ; Echeverría, G.; Lara, I. ML-G elaborated Supplementary Figure S1. doi: 10.1080/10412905.2001.9699646, Akakabe, Y., Kusunoki, A., Tanaka, R., and Kanetsune, Y. Boca Raton, FL: CRC Press. Fruit aroma is an important indicator to reflects the quality of fruit flavor. Characterization of bioactive compounds in Tunisian bitter orange (Citrus aurantium L.) peel and juice and determination of their antioxidant activities. Interestingly, the monoterpenoid isogeranial and the sesquiterpene α-santalene have been described only in C. aurantifolia (Feger et al., 2000; Costa et al., 2014), C. medica (Venturini et al., 2014; Mitropoulou et al., 2017) and C. limon (Vekiari et al., 2002; Cannon et al., 2015). Quantitatively, the most important difference with C. reticulata is for α-sinensal, since in C. reticulata this compound can reach a percentage above 0.7% (Lota et al., 2001b), while in C. sinensis its percentage does not rise of 0.05% (Njoroge et al., 2005a; Sawamura et al., 2005). ; Fernandez-Trujillo, J.P. Climacteric or non-climacteric behavior in melon fruit 1. In the case of these 10 species we considered that the number of studies already published is sufficient to rely on the total list of compounds identified as quite representative of their actual volatile profile. The major compound in C. bergamia is limonene, but its percentage can decrease down to 33% (Poiana et al., 2003). Unable to load your collection due to an error, Unable to load your delegates due to an error, Phenotypes, RIPENING INHIBITOR (RIN) protein content and ethylene (ET) response of wildâtype (WT) and RINâdeficient tomato (, Ethylene (ET) production and response of wildâtype (WT) and RIPENING INHIBITOR (RIN)âdeficient tomato fruits to propylene. Characterization of calamansi (Citrus microcarpa). The authors are grateful to Prof. Osman Ali Sidahmed (University of Gezira, Sudan) for assistance in reading through the manuscript and making appropriate amendment. The most abundant compounds are the same described in C. reticulata, also in very similar proportions. Ortiz, A.; Graell, J.; Lara, I. Molecules 13, 1333–1344. Also, in this species have been reported a relative large group of monoterpenoid compounds, being some of them almost exclusive compounds as limonene diepoxide (Högnadóttir and Rouseff, 2003; Njoroge et al., 2005a; Sawamura et al., 2005; Esquivel-Ferriño et al., 2014). Expression of genes was measured in fruits selected at the breaker (B), breaker + 5 (B + 5) and breaker + 10 (B + 10) stages. Due to the complex nature of the volatile profiles, volatile composition is continuously changing in fresh fruit. Investigation of sunlight-induced deterioration of aroma of pummelo (Citrus maxima) essential oil. Ibdah, M.; Azulay, Y.; Portnoy, V.; Wasserman, B.; Bar, E.; Meir, A.; Burger, Y.; Hirchberg, J.; Schaffer, A.A.; Katzir, N.; Tadmor, Y.; Lewinsohn, E. Functional characterization of. As in C. aurantifolia and in C. medica peel, copious mono- and sesquiterpene hydrocarbons have been reported in C. bergamia (Figure 2 and Supplementary Table S2). Bood, K.G. J. Agric. -. Si vous pensez qu'il manque des fruits dans cette liste vous pouvez cliquer-ici pour les ajouter!. Characterization of the odor-active volatiles in Citrus Hyuganatsu (Citrus tamurana Hort. (2011b). These results indicate the importance of a correct production and storage of Citrus essential oil to preserve its original freshness quality. Accessibility Essential oils from six herbal plants for biocontrol of the maixe weevil. Aroma characterization of tangerine hybrids by gas-chromatography-olfactometry and sensory evaluation. doi: 10.1021/jf040324x, Choi, H.-S. (2006). The Occurrence of Pentane-2-Thiol in Guava Fruit. The concentrated extract was analyzed by capillary GC and GC/MS. J. J. Essent. ; Wang, Y.J. ; Mattinson, D.S. Evol. doi: 10.1002/ffj.1658, Feger, W., Brandauer, H., and Ziegler, H. (2000). J. Agric. (2010). Davies KM, Hobson GE, Grierson D. 1988. Anal. Identification, synthesis, and characterization of novel sulfur-containing volatile compounds from the in-depth analysis of Lisbon lemon peels (Citrus limon L. Burm. Chemical biodiversity of the leaf and flower essential oils of Citrus aurantium L. from Dubrovnik area (Croatia) in comparison with Citrus sinensis L. Osbeck cv. Trouvé à l'intérieur – Page 119... le volatile paraît alors s'alléger malgré sa démarche raide et devient diffus , confus , gommé , proche de l'énigme , comme un souffle de poussière et de plume promené dans les trèfles et les fruits pourris , par l'ombre fraîche et ... Food Chem. ; Fahrasmane, L. Comparative study of free and glycoconjugated volatile compounds of three banana cultivars from French West Indies: Cavendish, Frayssinette and Plantain. Food Chem. ; Rosati, C. molecular biology research in Fragaria. Biotechnology Journal 8: 1144â1158. (2012). 2012 Mar;287(3):189-203. doi: 10.1007/s00438-011-0671-7. Boudhrioua, N.; Giampaoli, P.; Bonazzi, C. Changes in aromatic components of banana during ripening and air-drying. Fuggate, P.; Wongs-Aree, C.; Noichinda, S.; Kanlayanarat, S. Quality and volatile attributes of attached and detached “Pluk Mai Lie” papaya during fruit ripening. Rizzolo, A.; Grassi, M.; Zerbini, P.E. Most notably, C. grandis, C. paradisi, C. sinensis, and C. aurantium form a clade independent of another clade including C. medica and C. aurantifolia in both cluster analyses. doi: 10.1080/0972060X.2009.10643716, Wang, J., and Liu, Y. Chemical variability of the leaf oil of 113 hybrids from Citrus clementina (Commun) x Citrus deliciosa (Willow Leaf). Ramirez-Torres F, Ghogare R, Stowe E, Cerdá-Bennasser P, Lobato-Gómez M, Williamson-Benavides BA, Giron-Calva PS, Hewitt S, Christou P, Dhingra A. Transgenic Res. doi: 10.1002/ffj.1348, Verzera, A., Trozzi, A., Gazea, F., Cicciarello, G., and Cotroneo, A. Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm., and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterperne, DL-limonene. Biological lemon and sweet orange essential oil composition. Model outlining the role of ethylene (ET) and RIPENING INHIBITOR (RIN) in initiation and progression of climacteric ripening in tomato fruit. Technol. Using antisense RNA to study gene function: inhibition of carotenoid biosynthesis in transgenic tomatoes. Packaging and storage. Biotechnol. Different GC capillary columns have been used to analyze the volatile and semi-volatile Citrus compounds. ; Underwood, B.A. Unripe and commercially ripe fruits were placed in ripening chambers until complete ripening and compared to tree-ri … Influence of methyl jasmonate in conjunction with ethanol on the formation of volatile compounds in berries belonging to the Rosaceae. Food Chem. and Zanthoxylum tessmannii Engl. Plant Physiology 136: 2641â2651. (2003). créé par lili020401 le 5 Nov. 2014, validé par lareinelouise. doi: 10.1111/j.1365-2621.2012.03170.x, Karioti, A., Skaltsa, H., and Gbolade, A. doi: 10.1007/s00217-007-0781-y, Flamini, G., and Cioni, P. L. (2010). Effect of (−)- and (+)-methyl jasmonate on the formation of aroma-active esters in strawberry fruit. Along this review we have used one of the most common names for each compound, but all the different terms used in the scientific literature have been kept in Supplementary Table S2. Moreover, we used all the data of volatile compounds reviewed to perform a hierarchical clustering of these Citrus species based on the similarity of their rind volatile profiles. ; Allen, J.M. J. Shan, W.Y. This study indicates that oxygen and heating co-treatment increases the content of limonene oxides and carvone in pummelo EO. those of the individual authors and contributors and not of the publisher and the editor(s). B. An example of this is p-cymenene (Lota et al., 2002; Fanciullino et al., 2006; Pino et al., 2006), also named α-p-dimethylstyrene (Huang et al., 2000; Jabalpurwala et al., 2009; Cannon et al., 2015) and dehydro-4-cymene (Tomiyama et al., 2012). The number of taxonomic classifiers was narrow to eight (linalyl acetate, geranyl acetate, neryl acetate, β-cis-ocimene, δ-3-carene, β-myrcene, sabinene and α-terpineol). Tech. Essential oil of two new pigmented Citrus hybrids, Citrus clementina x Citrus sinensis. Food Chem. doi: 10.1556/AAlim.41.2012.1.15. Volatile organic compounds emitted from flowers have roles beyond pollinator attraction. ; Drawert, F.; Kollmannsberger, H. Geruchsaktive spurenkomponenten des bananen aromas. [. Find support for a specific problem in the support section of our website. Food Chem. Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme family. MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. ; Qin, X.; Loewen, M.C. show data from rind EOs (Dugo and Mondello, 2011). Volatile constituents in extracts of mandarin and tangerine peel. Du, X.F. Characterization of volatile compounds and aroma profiles of Malaysian pomelo (Citrus grandis (L.) Osbeck) blossom and peel. (2017). J. Agric. 46, 1455–1461. Therefore, we addressed the classification of these species by HCA based on their respective volatile profiles as described in the literature. Trouvé à l'intérieur – Page 374Le fragile labeur d'un insecte odieux , A tout un peuple volatile , Piége subcil & dangereux . Un guerrier de Juda , vi & time de la flamme ... D'un arbre toujours verd , le fruit âpre , incipide , si l'art n'en corrige l'acide . This approach allowed us to classify these species into three clusters based on their volatile profile as described in the literature (Figure 3) (Rambla et al., 2015). Non-terpenoid compounds very rarely represent more than 1%, although this does not necessarily mean that these compounds do not have an impact on the EO aroma. There are fewer studies about the VOCs in the flowers of Citrus most common species in comparation with Citrus rind. In vitro antimycoplasmal activity of Citrus bergamia essential oil and its major components. J. var. Composition of cold-pressed bitter orange oil from Cuba. Lipolytic effects of citrus peel oils and their components. J. Korean Soc. doi: 10.1111/j.1750-3841.2011.02421.x, Jing, L., Lei, Z., Li, L., Xie, R., Xi, R., Xi, W., et al. 14, 369–371. doi: 10.1016/j.foodchem.2013.12.070, Tomi, F., Barzalona, M., Casanova, J., and Luro, F. (2008). Phytochemistry 70, 1428–1434. Bot. Stumpe, M.; Feussner, I. (b) Water loss by WT and RINâCRISPR tomato fruits during postâharvest storage. Even some compounds have only been found in leaves, such as (Z)-2-penten-1-ol (Gancel et al., 2003, 2005) or (Z)-3-hexenyl butanoate (Flamini and Cioni, 2010). J. Hampel, D.; Mosandl, A.; Wust, M. Biosynthesis of mono- and sesquiterpenes in strawberry fruits and foliage: 2H labeling studies. Beaulieu, J.C. Effect of cutting and storage on acetate and nonacetate esters in convenient, ready-to-eat fresh-cut melons and apples. Antifungal activity of citrus essential oils. (Kiyookadaidai) cold-pressed peel oil. doi: 10.1002/jssc.200900267. doi: 10.14233/ajchem.2014.16277, Družić, J., Jerković, I., Marijanović, Z., and Roje, M. (2016). The leaf oils of the Australian species of Citrus (Rutaceae). by gas chromatography-olfactometry, aroma reconstitution, and omission test. (also C. maxima Burm. ; Mattheis, J.P.; Wyllie, S.G.; Fellman, J.K. 32, 3457–3465. The essential oils of leaves and fruit pericarps of Zanthoxylum leprieurii (Guill. This type of Additionally, the most widely used techniques for the extraction and analysis of volatile Citrus compounds are also described. 44, 544–549. These storage techniques and treatments involve cold, heat, irradiation, different storage atmospheres, and chemical applications. (2016) has reported some compounds in C. medica not described, or very rarely identified, in other Citrus species so far as like the monoterpenes cuminyl alcohol, dehydrosabina ketone or cis-4-caranone, and specially sesquiterpene like longifolene, 9-epi-caryophyllene, α-cuprenene, γ-cuprenene, italicene, nootkatol, or β-oplopenone, among many others. ; Martin, R.; Qian, M.C. of Citrus natsudaidai Hayata (Natsudaidai) cold-pressed peel oil. Ind. 13, 343–347. Fruit juice can be described as an unfermented product, but still can be fermented from mature, fresh, and good quality fruits (Bates et al., 2001). Please enable it to take advantage of the complete set of features! Variation in the components and antioxidant activity of Citrus medica L. var. All the common VOCs reported in the peel of the 10 Citrus species studies (Table 1) have also been identified in the leaves of most of these species, except perillaldehyde, only reported in C. limon (Guerrini et al., 2014) (Supplementary Table S2). 2021 Sep;134(9):2727-2747. doi: 10.1007/s00122-021-03874-3. Perry, P.L. Fresh tomato fruit flavour is the sum of the interaction between sugars, acids, and a set of approximately 30 volatile compounds synthesized from a diverse set of precursors, including amino acids . Fruit odor discrimination and sympatric host race formation in Rhagoletis. Turemis, N.; Kafkas, E.; Kafkas, S.; Kurkcuoglu, M.; Baser, K.H.C. ; Singh, Z.; Swinny, E.E. 2 to 6 players. ; Zhao, J.B.; Jiang, Q. ; Knee, M. Synthesis of aroma compounds by apples supplied with alcohols and methyl esters of fatty acids. Characteristic odour components of Citrus inflata Hort. Kerry, J. P., Kerry, J. F., and Ledward, D. (2002). ; Flores, G.; Ruiz del Castillo, M.L. Análisis no dirigido del aceite esencial de las variedades de mandarino Clemenules y Clemenpons. Flavor. La boite à énigmes . amara from Tunisia. 48, 1734–1740. (2011a). The other fiber used for Citrus oil and fresh flavedo was a 50 μm/30 μm DVB/CAR/PDMS fiber (Qiao et al., 2008; Mitropoulou et al., 2017), which is more polar than the other named fibers, and often used to extract volatile and semi-volatile compounds from the Citrus juices (González-Mas et al., 2011; Benjamin et al., 2013; Rambla et al., 2014; Qiu and Wang, 2015). 18, 74–79. (2012). [. is rich in chemical compounds of interest for the food and perfume industries, and therefore has been extensively studied during the last decades. Heatmap showing the relative frequency of each group of compounds is presented for each of the most common Citrus species. ex Tanaka (Omori et al., 2011), C. hystrix D.C. (kaffir lime) (Jirapakkul et al., 2013), Citrus sp. Many factors affect volatile composition, including the genetic makeup, degree of maturity, environmental conditions, postharvest handling and storage. 18, 275–281. Schwab, W.; Schaart, J.G. Trouvé à l'intérieur – Page 170Cette substance grasse est une huile volatile plus pesante que l'eau ; son odeur est extrêmement pénétrante et sa ... En effet , si ces deux substances existaient déjà toutes formées dans le fruit , comme l'essence de térébenthine dans ... J. Essent. (2014). Chem. (2018). Essential oils from the Malaysian Citrus (Rutaceae) medicinal plants. In this study, we have particularly focussed on the presence of volatile compounds in the peel of the 10 Citrus species most commonly analyzed, for which a significant amount of information is available in the scientific literature. ; Sheng, H.L. ; Moreno, E.; Garcıa-Mas, J.; Nicolai, B.; Lammertync, J.; Monforte, J.A. Various chemical and biological controls . Chemical composition and in vitro antimicrobial, cytotoxic, and central nervous system activities of the essential oils of Citrus medica L. cv. Influence of ethylene removal on the volatile development in. Choi, H.-S. (2003a). ; Bartolomé, B.; Ruiz del Castillo, M.L. ; Rambla, J.L. ; Lara, I. This is the case of the compound (Z)-9-dodecen-12-olide, also named yuzu lactone or oxacyclotridec-10-en-2-one, only isolated in C. junos (Miyazawa et al., 2009; Tomiyama et al., 2012) and C. limon (Cannon et al., 2015), or the compound decanoic acid, identified in C. junos (Song et al., 2000b; Tomiyama et al., 2012), C. grandis (Cheong et al., 2011b), C. limon (Cannon et al., 2015), C. reticulata (Pino and Quijano-Celís, 2007) and C. aurantium (Dugo and Mondello, 2011). J. Essent. doi: 10.1007/s13765-012-0023-2, Chung, M. S. (2012). (2002) [7] reported that the threshold level (in cookies) of the volatile oils of cardamom and cinnamon was 0.05%, while that for clove volatile oil was 0.075%. Comparison of free and glycosidically linked volatile components from polyembryonic and monoembryonic mango (, Aubert, C.; Milhet, C. Distribution of the volatile compounds in the different parts of a white-fleshed peach (. Profiles of essential oils of peel and leaf of a new Citrus hybrid, Citrus latifolia Tanaka x Citrus aurantifolia Swingle. ; Fang, J.B.; Li, S.H. (2012). Oil Res. ; Matich, A.J. Molecules 18, 10639–10647. Sains Malays. Simkin, A.J. Rizzolo, A.; Cambiaghi, P.; Grassi, M.; Zerbini, P.E. Seasonal variation in lemon (Citrus limon L. Burm. Citrus Oils: Composition, Advanced Analytical Techniques, Contaminants, and Biological Activity, Vol. Flavour Frag. Clementine using gas chromatography-olfactometry. Contribution of volatile compounds to mango (, Quijano, C.E. Storage temperature is a fundamental factor affecting the flavor of fruits. Table 1 Volatile organic compounds for banana plants. These compounds are hexanal, octanal, and decanal self-aldol condensation products, respectively. To prevent confusion, an organic extract from Citrus peel should not be named EO, (Alissandrakis et al., 2003; Chisholm et al., 2003b) although it could simply be named oil (Feger et al., 2001b; Buettner et al., 2003; Craske et al., 2005; Fisher et al., 2008). Evaluation of the most odour-active compounds in the peel oil of clementines (Citrus reticulata blanco cv. Femminello comune. doi: 10.1016/j.indcrop.2015.07.019, Bonaccorsi, I. L., McNair, H. M., Brunner, L. A., Dugo, P., and Dugo, G. (1999). Trouvé à l'intérieurLe fruit que porte N'aimeroit que trop bien , si je le laifois par impuissance arrivée par incendie , guerre , le hêtre , qui est une clpece ... Faire une énigme . ... volatile ; & une faim Ludovicus nihil faciens , Louis le faineant . J. Agric. Quantification of selected aroma-active compounds in strawberries by headspace solid-phase microextraction gas chromatography and correlation with sensory descriptive analysis. Most of the VOCs identified in any of these Citrus species have also been isolated in C. reticulata. Therefore, we have used a conservative approach all along this review for the description of the volatile profiles, as well as for the generation of figures and statistical analyses.
fruit et volatile enigme 2021